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DRX486703: Deep-sequencing of SAMD00642683
1 ILLUMINA (Illumina MiSeq) run: 142,230 spots, 39.5M bases, 23.4Mb downloads

Submitted by: KUMAMOTO
Study: Vulnerability to APOBEC3G linked to the pathogenicity of deltaretroviruses
show Abstracthide Abstract
Mutational analysis of provirus using samples from HTLV-1 carriers, HTLV-2-infected individuals, and STLV-1-infected Japanese macaques. Further, RNA-seq and ChIP-seq demonstrated that activation of TGF-beta is important for ATL.
Sample: HTLV-1_S25
SAMD00642683 • DRS347811 • All experiments • All runs
Library:
Name: HTLV-1_carrier_S25
Instrument: Illumina MiSeq
Strategy: Targeted-Capture
Source: GENOMIC
Selection: size fractionation
Layout: PAIRED
Construction protocol: Genomic DNA from HTLV-1 carriers, HTLV-2 infected individuals, and STLV-1 infected Japanese macaques was extracted with phenol-chloroform. To amplify the whole provirus sequences of HTLV-1, HTLV-2, and STLV-1, long-PCR was performed. The libraries were prepared using the Nextera XT DNA Library Preparation Kit (Illumina), according to the manufacturer's instructions. Then deep-sequencing analysis was performed with MiSeq (Illumina).
Pipeline: show...hide...
StepPrev Step
1NIL
Runs: 1 run, 142,230 spots, 39.5M bases, 23.4Mb
Run# of Spots# of BasesSizePublished
DRR502708142,23039.5M23.4Mb2023-12-31

ID:
31150228

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